Replaying bacterial evolution from a freezer
In a 2008 E. coli study, researchers revived archived bacteria to test whether the later ability to use citrate in oxygenated conditions depended on changes that had already occurred.
Questions you could explore
- What does replaying from several starting generations reveal that watching a single lineage cannot?
- How could comparing replayed bacterial populations test whether an earlier mutation made a later change more likely?
Start reading
- PNAS / Blount Lab author copyBlount, Borland and Lenski (2008), PNAS, DOI 10.1073/pnas.0803151105
The original paper explains the frozen archive and experiments testing whether earlier history made a later innovation more likely.
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